MolNFT v1 · #180575 · protein + DNA + RNA
Cryo-EM structure of CRISPR-CAS12F ternary complex
7L49 · CAS12F1: “Cryo-EM structure of CRISPR-CAS12F ternary complex”. Electron microscopy, 1 ligand (ZN), 20 pockets (best druggability 0.79). MolNFT v1 #180575 on GenesisL1: 3D structure, binding sites and pockets in GL1X.
- PDB
- 7L49
- Type
- protein + DNA + RNA
- Length
- 1,231 residues
- Chains
- 6
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- CAS12F1; Ts; Nts; Sgrna; Substrate
- Ligands
- ZN
- Pockets
- 20 · best druggability 0.79
Structure
electron microscopy · 6 distinct chains · 40% helix, 14% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | CAS12F1 | protein | 522 | 40% helix, 15% strand | — |
| B | CAS12F1 | protein | 519 | 39% helix, 13% strand | — |
| C | Ts | dna | 29 | — | — |
| D | Nts | dna | 21 | — | — |
| E | Sgrna | rna | 135 | — | — |
| F | Substrate | dna | 5 | — | — |
Ligands and binding sites
- ZN · Zinc ion · metal · 4 copies · chains A, B
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 15 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 270 | 0.79 | 0.92 | 33 | empty |
| 2 | 360 | 0.77 | 0.88 | 36 | empty |
| 3 | 611 | 0.75 | 0.83 | 43 | empty |
| 4 | 325 | 0.74 | 0.87 | 34 | empty |
| 5 | 266 | 0.71 | 0.86 | 37 | empty |
Interfaces
10 interfaces between chains.
- A–E · 4572 Ų buried · 87 hydrogen bonds · protein-nucleic
- B–E · 2215 Ų buried · 31 hydrogen bonds · protein-nucleic
- A–B · 1444 Ų buried · 7 hydrogen bonds · protein-protein
- A–C · 1307 Ų buried · 21 hydrogen bonds · protein-nucleic
- A–D · 1125 Ų buried · 16 hydrogen bonds · protein-nucleic
Compared structures
- 7L49 · Cryo-EM structure of CRISPR-Cas12f Ternary Complex · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Elsewhere: RCSB PDB 7L49 · PDBe 7L49
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