Crystal structure of the lrr ectodomain of the plant membrane receptor kinase GASSHO1/SCHENGEN3 from arabidopsis thaliana in complex with casparian strip integrity factor 2.

Structure

x-ray diffraction · 2 distinct chains · 19% helix, 23% strand (DSSP).

Ligands and binding sites

• NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 33 copies · chains A, C, B, D
• BMA · beta-D-mannopyranose · sugar · 3 copies

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

3 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.

Interfaces

3 interfaces between chains.

• A–C · 1374 Ų buried · 22 hydrogen bonds · protein-protein
• B–D · 1367 Ų buried · 19 hydrogen bonds · protein-protein
• A–B · 174 Ų buried · 5 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 27 glycan, 6 disulfide, 2 modified (N-glycosylation, disulfide, sulfation).

Compared structures

• 6S6Q · Crystal structure of the LRR ectodomain of the plant membrane receptor kinase… · the same entry in another collection · chain D against D, TM-score 1.00
• C0LGQ5 · AlphaFold monomer V2.0 prediction for lrr receptor-like SERINE/THREONINE-protein kinase… · a predicted model of the same protein · chain A against A, TM-score 0.97
• O65684 · AlphaFold monomer V2.0 prediction for protein casparian strip integrity factor 2 (O65684) · a predicted model of the same protein · chain D against A, TM-score 0.22

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt C0LGQ5
• Every structure of UniProt O65684
• Elsewhere: RCSB PDB 6S6Q · PDBe 6S6Q · UniProt C0LGQ5 · UniProt O65684