MolNFT v1 · #160383 · protein

Mouse two pore domain K+ channel traak (K2P4.1) - Fab complex structure

6PIS · Potassium channel subfamily K member 4: “Mouse two pore domain K+ channel traak (K2P4.1) - Fab complex structure”. X-ray diffraction, 1 ligand (K), 18 pockets (best druggability 0.70). MolNFT v1 #160383 on GenesisL1: 3D structure…

PDB: 6PIS
Type: protein
Length: 1,325 residues
Chains: 6
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Potassium channel subfamily K member 4; Antibody Fab fragment light chain; Antibody Fab…
UniProt: O88454
Ligands: K
Pockets: 18 · best druggability 0.70

Structure

x-ray diffraction · 5 distinct chains · 37% helix, 30% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Potassium channel subfamily K member 4	protein	235	77% helix, 0% strand	O88454
B	Potassium channel subfamily K member 4	protein	239	78% helix, 0% strand	O88454
L, M	Antibody Fab fragment light chain	protein	211	7% helix, 51% strand	—
H	Antibody Fab fragment heavy chain	protein	215	6% helix, 52% strand	—
I	Antibody Fab fragment heavy chain	protein	214	6% helix, 52% strand	—

Ligands and binding sites

K · Potassium ion · metal · 6 copies · chains B, A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

18 pockets found on a 1 Å grid, 8 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	142	0.70	0.89	25	empty
2	316	0.70	0.83	46	empty
3	782	0.69	0.76	77	empty
4	573	0.68	0.77	38	K
5	685	0.64	0.74	69	K

Interfaces

11 interfaces between chains.

A–B · 5137 Å² buried · 41 hydrogen bonds · protein-protein
I–M · 1837 Å² buried · 14 hydrogen bonds · protein-protein
L–H · 1831 Å² buried · 15 hydrogen bonds · protein-protein
A–M · 430 Å² buried · 5 hydrogen bonds · protein-protein
B–H · 387 Å² buried · 7 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 9 disulfide (disulfide).

Compared structures

6PIS · Mouse two pore domain K+ channel TRAAK (K2P4.1) - Fab complex structure · the same entry in another collection · chain M against M, TM-score 1.00
O88454 · AlphaFold monomer V2.0 prediction for potassium channel subfamily K member 4 (O88454) · a predicted model of the same protein · chain B against A, TM-score 0.94

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt O88454
Elsewhere: RCSB PDB 6PIS · PDBe 6PIS · UniProt O88454

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