MolNFT v1 · #142534 · protein
Crystal structure of the fifth immunoglobulin domain (IG5) of human ROBO1 in complex with the Fab fragment of murine monoclonal antibody B5209B
6A77 · Roundabout homolog 1: “Crystal structure of the fifth immunoglobulin domain (IG5) of human ROBO1 in complex with the Fab fragment of…”. X-ray diffraction, 6 pockets (best druggability 0.72). MolNFT v1 #142534 on GenesisL1: 3D…
- PDB
- 6A77
- Type
- protein
- Length
- 519 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Roundabout homolog 1; Light chain of the anti-human ROBO1 antibody B5209B Fab; Heavy…
- UniProt
- Q9Y6N7
- Pockets
- 6 · best druggability 0.72
Structure
x-ray diffraction · 3 distinct chains · 7% helix, 54% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Roundabout homolog 1 | protein | 91 | 3% helix, 54% strand | Q9Y6N7 |
| L | Light chain of the anti-human ROBO1 antibody B5209B Fab | protein | 211 | 6% helix, 55% strand | — |
| H | Heavy chain of the anti-human ROBO1 antibody B5209B Fab | protein | 217 | 8% helix, 53% strand | — |
Pockets
6 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 846 | 0.72 | 0.77 | 71 | empty |
| 2 | 134 | 0.49 | 0.79 | 20 | empty |
| 3 | 113 | 0.42 | 0.77 | 27 | empty |
| 4 | 96 | 0.38 | 0.76 | 23 | empty |
| 5 | 71 | 0.32 | 0.75 | 18 | empty |
Interfaces
3 interfaces between chains.
- L–H · 1823 Ų buried · 12 hydrogen bonds · protein-protein
- A–H · 495 Ų buried · 6 hydrogen bonds · protein-protein
- A–L · 402 Ų buried · 7 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 5 disulfide (disulfide).
Compared structures
- 6A77 · Crystal structure of the fifth immunoglobulin domain (Ig5) of human Robo1 in complex with… · the same entry in another collection · chain A against A, TM-score 1.00
- Q9Y6N7 · AlphaFold monomer V2.0 prediction for roundabout homolog 1 (Q9Y6N7) · a predicted model of the same protein · chain A against A, TM-score 0.94
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q9Y6N7
- Elsewhere: RCSB PDB 6A77 · PDBe 6A77 · UniProt Q9Y6N7
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.