MolNFT v1 · #135961 · protein

Structure of antigen-Fab 12E complex with histone chaperone ASF1

5UEK: “Structure of antigen-Fab 12E complex with histone chaperone ASF1”. X-ray diffraction, 5 pockets (best druggability 0.52). MolNFT v1 #135961 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB: 5UEK
Type: protein
Length: 579 residues
Chains: 3
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Histone chaperone ASF1; Fab 12E heavy chain; Fab 12E light chain
UniProt: P32447
Pockets: 5 · best druggability 0.52

Structure

x-ray diffraction · 3 distinct chains · 8% helix, 53% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Histone chaperone ASF1	protein	153	10% helix, 52% strand	P32447
H	Fab 12E heavy chain	protein	217	7% helix, 54% strand	—
L	Fab 12E light chain	protein	209	7% helix, 51% strand	—

Pockets

5 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	291	0.52	0.74	34	empty
2	136	0.42	0.75	27	empty
3	93	0.37	0.76	28	empty
4	62	0.32	0.75	20	empty
5	56	0.25	0.72	18	empty

Interfaces

3 interfaces between chains.

H–L · 1868 Å² buried · 11 hydrogen bonds · protein-protein
A–H · 740 Å² buried · 8 hydrogen bonds · protein-protein
A–L · 648 Å² buried · 17 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 4 disulfide (disulfide).

Compared structures

5UEK · Structure of antigen-Fab 12E complex with Histone chaperone ASF1 · the same entry in another collection · chain A against A, TM-score 1.00
P32447 · AlphaFold monomer V2.0 prediction for histone chaperone ASF1 (P32447) · a predicted model of the same protein · chain A against A, TM-score 0.95

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P32447
Elsewhere: RCSB PDB 5UEK · PDBe 5UEK · UniProt P32447

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