MolNFT v1 · #128826 · protein

E. COLI fold apo

5O28 · Bifunctional protein fold: “E. COLI fold apo”. X-ray diffraction, 12 pockets (best druggability 0.74). MolNFT v1 #128826 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB: 5O28
Type: protein
Length: 1,139 residues
Chains: 4
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Bifunctional protein fold
EC: 1.5.1.5, 3.5.4.9
UniProt: P24186
Pockets: 12 · best druggability 0.74

Structure

x-ray diffraction · 3 distinct chains · 43% helix, 16% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Bifunctional protein fold (EC 1.5.1.5, 3.5.4.9)	protein	285	44% helix, 16% strand	P24186
B, D	Bifunctional protein fold (EC 1.5.1.5, 3.5.4.9)	protein	285	42% helix, 16% strand	P24186
C	Bifunctional protein fold (EC 1.5.1.5, 3.5.4.9)	protein	284	41% helix, 16% strand	P24186

Pockets

12 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	777	0.74	0.79	76	empty
2	447	0.63	0.76	59	empty
3	343	0.62	0.78	30	empty
4	369	0.60	0.76	50	empty
5	274	0.55	0.76	36	empty

Interfaces

5 interfaces between chains.

A–B · 1692 Å² buried · 32 hydrogen bonds · protein-protein
C–D · 1466 Å² buried · 24 hydrogen bonds · protein-protein
B–C · 242 Å² buried · 0 hydrogen bonds · protein-protein
B–D · 170 Å² buried · 3 hydrogen bonds · protein-protein
A–D · 19 Å² buried · 0 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 8 modified (methylation).

Compared structures

5O28 · E. coli FolD apo · the same entry in another collection · chain D against D, TM-score 1.00
P24186 · AlphaFold monomer V2.0 prediction for bifunctional protein fold (P24186) · a predicted model of the same protein · chain B against A, TM-score 0.96

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P24186
Elsewhere: RCSB PDB 5O28 · PDBe 5O28 · UniProt P24186

GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.