MolNFT v1 · #124838 · protein

Directed evolution of transaminases by ancestral reconstruction. using old proteins for new chemistries

5KR3 · 4-aminobutyrate transaminase: “Directed evolution of transaminases by ancestral reconstruction. using old proteins for new chemistries”. X-ray diffraction, 1 ligand (PLP), 8 pockets (best druggability 0.75). MolNFT v1 #124838 on…

PDB: 5KR3
Type: protein
Length: 916 residues
Chains: 2
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: 4-aminobutyrate transaminase
EC: 2.6.1.18
Ligands: PLP
Pockets: 8 · best druggability 0.75

Structure

x-ray diffraction · 2 distinct chains · 45% helix, 18% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	4-aminobutyrate transaminase (EC 2.6.1.18)	protein	458	45% helix, 18% strand	—
B	4-aminobutyrate transaminase (EC 2.6.1.18)	protein	458	45% helix, 18% strand	—

Ligands and binding sites

PLP · Pyridoxal-5'-phosphate · ligand · 2 copies · chains A, B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

8 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	543	0.75	0.82	64	PLP
2	642	0.74	0.81	79	PLP
3	109	0.41	0.76	16	empty
4	111	0.38	0.74	24	empty
5	68	0.32	0.75	18	empty

Interfaces

1 interface between chains.

A–B · 5550 Å² buried · 83 hydrogen bonds · protein-protein

Compared structures

5KR3 · Directed Evolution of Transaminases By Ancestral Reconstruction. Using Old Proteins for… · the same entry in another collection · chain A against A, TM-score 1.00

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Elsewhere: RCSB PDB 5KR3 · PDBe 5KR3

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