Crystal structure of the E. COLI type 1 pilus subunit fimg (engineered variant with substitution Q134E; N-terminal fimg residues 1-12 truncated) in complex with the donor strand peptide DSF_T4R-T6R-D13N

Structure

x-ray diffraction · 3 distinct chains · 3% helix, 54% strand (DSSP).

Ligands and binding sites

• CO · Cobalt (II) ion · metal · 4 copies · chains G, A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

2 pockets found on a 1 Å grid, 0 with a druggability of 0.5 or more.

Interfaces

5 interfaces between chains.

• A–B · 1306 Ų buried · 43 hydrogen bonds · protein-protein
• G–F · 1207 Ų buried · 32 hydrogen bonds · protein-protein
• G–A · 606 Ų buried · 7 hydrogen bonds · protein-protein
• A–F · 126 Ų buried · 4 hydrogen bonds · protein-protein
• G–B · 123 Ų buried · 4 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 1 disulfide (disulfide).

Compared structures

• 5IQM · Crystal structure of the E. coli type 1 pilus subunit FimG (engineered variant with… · the same entry in another collection · chain B against B, TM-score 1.00
• P08190 · AlphaFold monomer V2.0 prediction for protein fimg (P08190) · a predicted model of the same protein · chain G against A, TM-score 0.99
• P08189 · AlphaFold monomer V2.0 prediction for protein fimf (P08189) · a predicted model of the same protein · chain F against A, TM-score 0.59

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P08190
• Every structure of UniProt P08189
• Elsewhere: RCSB PDB 5IQM · PDBe 5IQM · UniProt P08190 · UniProt P08189