MolNFT v1 · #122110 · protein
Crystal structure of higa-higb complex from E. COLI
5IFG · MRNA interferase higb: “Crystal structure of higa-higb complex from E. COLI”. X-ray diffraction, 8 pockets (best druggability 0.43). MolNFT v1 #122110 on GenesisL1: 3D structure, binding sites and pockets in GL1X.
- PDB
- 5IFG
- Type
- protein
- Length
- 435 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- MRNA interferase higb; Antitoxin higa
- EC
- 3.1.-.-
- UniProt
- P64578, P67701
- Pockets
- 8 · best druggability 0.43
Structure
x-ray diffraction · 4 distinct chains · 54% helix, 12% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | MRNA interferase higb (EC 3.1.-.-) | protein | 100 | 42% helix, 27% strand | P64578 |
| B | Antitoxin higa | protein | 118 | 66% helix, 0% strand | P67701 |
| C | MRNA interferase higb (EC 3.1.-.-) | protein | 98 | 40% helix, 28% strand | P64578 |
| D | Antitoxin higa | protein | 119 | 66% helix, 0% strand | P67701 |
Pockets
8 pockets found on a 1 Å grid, 0 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 184 | 0.43 | 0.73 | 27 | empty |
| 2 | 114 | 0.40 | 0.75 | 16 | empty |
| 3 | 56 | 0.37 | 0.80 | 17 | empty |
| 4 | 80 | 0.37 | 0.77 | 17 | empty |
| 5 | 89 | 0.37 | 0.75 | 21 | empty |
Interfaces
3 interfaces between chains.
- C–D · 1489 Ų buried · 14 hydrogen bonds · protein-protein
- A–B · 1466 Ų buried · 13 hydrogen bonds · protein-protein
- B–D · 1129 Ų buried · 2 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 14 modified (engineered).
Compared structures
- 5IFG · Crystal structure of HigA-HigB complex from E. Coli · the same entry in another collection · chain D against D, TM-score 1.00
- P64578 · AlphaFold monomer V2.0 prediction for mRNA interferase toxin higb (P64578) · a predicted model of the same protein · chain C against A, TM-score 0.98
- P67701 · AlphaFold monomer V2.0 prediction for antitoxin higa (P67701) · a predicted model of the same protein · chain D against A, TM-score 0.89
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P64578
- Every structure of UniProt P67701
- Elsewhere: RCSB PDB 5IFG · PDBe 5IFG · UniProt P64578 · UniProt P67701
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