MolNFT v1 · #119390 · protein
Extending the half-life of a Fab fragment through generation of a humanised anti-human serum albumin (hsa) fv domain: an investigation into the correlation between affinity and serum half-life
5FUO: “Extending the half-life of a Fab fragment through generation of a humanised anti-human serum albumin (hsa) fv…”. X-ray diffraction, 11 pockets (best druggability 0.84). MolNFT v1 #119390 on GenesisL1: 3D structure, binding sites and…
- PDB
- 5FUO
- Type
- protein
- Length
- 1,011 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Serum albumin; Fab heavy chain; Fab light chain
- UniProt
- P02768
- Pockets
- 11 · best druggability 0.84
Structure
x-ray diffraction · 3 distinct chains · 42% helix, 21% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Serum albumin | protein | 578 | 70% helix, 0% strand | P02768 |
| H | Fab heavy chain | protein | 216 | 6% helix, 48% strand | — |
| L | Fab light chain | protein | 217 | 6% helix, 50% strand | — |
Pockets
11 pockets found on a 1 Å grid, 7 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1152 | 0.84 | 0.85 | 113 | empty |
| 2 | 1151 | 0.78 | 0.80 | 104 | empty |
| 3 | 475 | 0.58 | 0.74 | 51 | empty |
| 4 | 159 | 0.57 | 0.81 | 18 | empty |
| 5 | 108 | 0.57 | 0.84 | 25 | empty |
Interfaces
3 interfaces between chains.
- H–L · 1918 Ų buried · 18 hydrogen bonds · protein-protein
- A–H · 342 Ų buried · 6 hydrogen bonds · protein-protein
- A–L · 303 Ų buried · 3 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 21 disulfide (disulfide).
Compared structures
- 5FUO · Extending the half-life of a Fab fragment through generation of a humanised anti-Human… · the same entry in another collection · chain A against A, TM-score 1.00
- P02768 · AlphaFold monomer V2.0 prediction for albumin (P02768) · a predicted model of the same protein · chain A against A, TM-score 0.98
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P02768
- Elsewhere: RCSB PDB 5FUO · PDBe 5FUO · UniProt P02768
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