Reversible S-nitrosylation in an engineered mutant of pseudomonas aeruginosa azurin with red copper site

Structure

x-ray diffraction · 1 distinct chain · 19% helix, 33% strand (DSSP).

Ligands and binding sites

• CU · Copper (II) ion · metal · 4 copies · chains A, B
• TRS · 2-amino-2-hydroxymethyl-propane-1,3-diol · additive · 2 copies · chains A, B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

2 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–B · 188 Ų buried · 2 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 2 disulfide (disulfide).

Compared structures

• 4WKX · Reversible S-Nitrosylation in an Engineered Mutant of Pseudomonas aeruginosa Azurin with… · the same entry in another collection · chain A against A, TM-score 1.00
• P00282 · AlphaFold monomer V2.0 prediction for azurin (P00282) · a predicted model of the same protein · chain B against A, TM-score 0.97

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P00282
• Elsewhere: RCSB PDB 4WKX · PDBe 4WKX · UniProt P00282