MolNFT v2 · #224162 · protein
8WKI: Cryo-EM structure of the distal rod-hook within the flagellar motor-hook complex in the CW state.
8WKI · Flagellar hook protein FlgE: “Cryo-EM structure of the distal rod-hook within the flagellar motor-hook complex in the CW state.”. Electron microscopy at 3.3, 20 pockets (best druggability 0.89). MolNFT v2 #224162 on GenesisL1: 3D…
- PDB
- 8WKI
- Type
- protein
- Length
- 17,770 residues
- Chains
- 53
- Method
- electron microscopy
- Authors
- Tan, J.X., Zhang, L., Zhou, Y., Zhu, Y.Q.
- Deposited
- 09/27/23
- Collection
- MolNFT v2 / PDB Bank
- Contract
- 0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
- Owner
- 0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
- Resolution
- 3.3
- Source
- Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
- Molecule
- Flagellar hook protein FlgE; Flagellar basal-body rod protein FlgG
- UniProt
- P0A1J1, P0A1J3
- Pockets
- 20 · best druggability 0.89
Structure
electron microscopy · resolution 3.3 · 8 distinct chains · 23% helix, 33% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| ZF, ZG, ZH, ZI, ZJ, ZK, ZL, ZM, ZN, ZO, ZP, ZQ, ZR, ZS, ZT, ZU, ZV, ZW, ZX, ZY, ZZ, Za, Zb, Zc, Zd, Ze, Zf, Zg, Zh | Flagellar hook protein FlgE | protein | 401 | 15% helix, 40% strand | P0A1J1 |
| 2, 3, 4, 5, 6, 7, 8, 9, ZA, ZB, ZC, ZD, ZE | Flagellar basal-body rod protein FlgG | protein | 260 | 23% helix, 29% strand | P0A1J3 |
| 0, x, y, z | Flagellar basal-body rod protein FlgG | protein | 248 | 24% helix, 32% strand | P0A1J3 |
| 1 | Flagellar basal-body rod protein FlgG | protein | 252 | 25% helix, 33% strand | P0A1J3 |
| r, u | Flagellar basal-body rod protein FlgG | protein | 254 | 25% helix, 30% strand | P0A1J3 |
| s, v | Flagellar basal-body rod protein FlgG | protein | 255 | 25% helix, 30% strand | P0A1J3 |
| t | Flagellar basal-body rod protein FlgG | protein | 256 | 25% helix, 34% strand | P0A1J3 |
| w | Flagellar basal-body rod protein FlgG | protein | 243 | 24% helix, 31% strand | P0A1J3 |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1175 | 0.89 | 0.90 | 136 | empty |
| 2 | 993 | 0.89 | 0.90 | 122 | empty |
| 3 | 1170 | 0.88 | 0.90 | 165 | empty |
| 4 | 974 | 0.88 | 0.90 | 95 | empty |
| 5 | 1159 | 0.88 | 0.90 | 128 | empty |
Interfaces
182 interfaces between chains.
- 2–7 · 2737 Ų buried · 32 hydrogen bonds · protein-protein
- 1–w · 2701 Ų buried · 21 hydrogen bonds · protein-protein
- 5–0 · 2698 Ų buried · 27 hydrogen bonds · protein-protein
- 3–8 · 2692 Ų buried · 29 hydrogen bonds · protein-protein
- 2–x · 2682 Ų buried · 27 hydrogen bonds · protein-protein
Compared structures
- P0A1J3 · AlphaFold monomer V2.0 prediction for flagellar basal-body rod protein flgg (P0A1J3) · a predicted model of the same protein · chain 7 against A, TM-score 0.97
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P0A1J1
- Every structure of UniProt P0A1J3
- Elsewhere: RCSB PDB 8WKI · PDBe 8WKI · UniProt P0A1J1 · UniProt P0A1J3
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